Cleavage of nucleic acid acid using thermostable methoanococcus jannaschii FEN-1 endonucleases
US5843669A · kind A · utility
219Cited by
9References
26Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 29, 1996 |
| Grant date | Dec 1, 1998 |
| Priority date | — |
| Expiry date | Nov 29, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/822
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Structure-specific nucleases, including 5' nucleases, thermostable FEN-1 endonucleases and 3' exonucleases, are used to detect and identify target nucleic acids. Methods are provided which allow for the detection specific nucleic acid sequences; these methods permit the detection and identification of mutant and wild-type forms of genes (e.g., human genes) as well as permit the detection and identification of bacterial and viral pathogens in a sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.