Dioxetane compounds for the chemiluminescent detection of proteases, methods of use and kits therefore
US5843681A · kind A · utility
5Cited by
11References
16Claims
0Family size
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Key dates
| Filing date | Oct 11, 1996 |
| Grant date | Dec 1, 1998 |
| Priority date | — |
| Expiry date | Oct 11, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/807
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
1, 2-dioxetane compounds bearing a proteolytic enzyme specific amino acid or peptide are provided, which amino acid or peptide can be removed by action of the corresponding protease. When the amino acid or peptide is removed, the 1,2-dioxetane decomposes with chemiluminescence, the generation of light providing a rapid, ultra-sensitive and convenient means for detecting the presence of the protease in the sample being inspected. The amount of light generated, or degree of chemiluminescence, can be correlated with the amount of protease present. Immunoassays, as well as DNA hybridization and DNA probe assays are provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.