Enhanced production of toxic polypeptides in prokaryotes
US5843703A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 25, 1994 |
| Grant date | Dec 1, 1998 |
| Priority date | — |
| Expiry date | Aug 25, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2795/10222
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Compositions for enhancing the production of toxic polypeptides in prokaryotic hosts comprise a coding nucleic acid and a bacteriophage nucleic acid. The coding nucleic acid encodes a promoter operably linked to a gene encoding a selected toxic protein. The bacteriophage nucleic acid contains at least one gene from the early region of a T7-like bacteriophage other than an RNA polymerase. When both the coding nucleic acid and the bacteriophage nucleic acid are used to transform or transfect a prokaryotic host, the production of the toxic polypeptide is greater than the level of production of the same toxic polypeptide when the same host is transformed only with the coding nucleic acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.