Expression of a cleavable fusion protein comprising a soluble human erythropoietin receptor protein fragment
US5843726A · kind A · utility
Inventor
Key dates
| Filing date | May 5, 1997 |
| Grant date | Dec 1, 1998 |
| Priority date | — |
| Expiry date | May 5, 2017 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/71
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An E. coli recombinant plasmid expressing a fusion protein having the human erythropoietin receptor extracellular domain is disclosed. A purified fusion protein produced from such a vector is also disclosed, the fusion protein having a cleavage site suitable for separating the erythropoietin receptor extracellular domain from the remainder of the fusion protein. Antibodies having specific binding affinity for a purified extracellular domain polypeptide are also disclosed. The purified human erythropoietin receptor fragment polypeptide binds erythropoietin. The articles, compositions and methods of the invention are useful for studying ligand binding to erythropoietin receptor and for quantitating the amounts of erythropoietin receptor, as well as for understanding receptor structure and signal transduction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.