Biotinated firefly luciferase, a gene for biotinated firefly luciferase, a recombinant DNA, a process for producing biotinated luciferase and a bioluminescent analysis method
US5843746A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 13, 1997 |
| Grant date | Dec 1, 1998 |
| Priority date | — |
| Expiry date | Jan 13, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to biotinated firefly luciferase comprising a biotinated peptide and firefly luciferase linked therein, biotinated firefly luciferase having a specific amino acid sequence, a biotinated firefly luciferase gene comprising a gene coding for a biotinated peptide and a firefly luciferase gene linked therein, a biotinated firefly luciferase gene comprising a biotinated peptide gene coding for a specific amino acid sequence and a firefly luciferase gene linked therein, a recombinant DNA comprising said biotinated firefly luciferase gene inserted into a vector DNA, a process for producing biotinated firefly luciferase comprising culturing a microorganism belonging to the genus Escherichia carrying said recombinant DNA and then recovering the resulting biotinated firefly luciferase from the culture, a bioluminescent analysis method comprising using said biotinated firefly luciferase, and a bioluminescent analysis method comprising quantifying a ligand by measuring a biotinated receptor by the use of said biotinated firefly luciferase. According to the present invention, there are provided biotinated firefly luciferase, a biotinated firefly luciferase gene, a r…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.