Patent · US Expired

Apparatus for the growth and packaging of three dimensional tissue cultures

US5843766A · kind A · utility

58Cited by
25References
63Claims
0Family size

Assignee

Inventors

Key dates

Filing dateFeb 5, 1996
Grant dateDec 1, 1998
Priority date
Expiry dateFeb 5, 2016

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12M29/10
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

A tissue culture chamber is disclosed. The chamber is a casing that provides for growth of three-dimensional tissue, and more specifically for the growth of skin tissue, that can be grown, preserved in frozen form, and shipped to the end user in the same aseptic container. The tissue culture chamber includes a casing comprising a substrate within the casing designed to facilitate three-dimensional tissue growth on the surface of the substrate. The casing includes an inlet and an outlet port which assist the inflow and outflow of media. The casing also includes at least one flow distributor. In one embodiment, the flow distributor is a baffle, which is used to distribute the flow of the media within the chamber to create a continuous, uniform piece of three-dimensional tissue. In a second embodiment, the flow distributor is a combination of deflector plates, distribution channels, and a flow channel. In each embodiment, the casing further includes a seal so as to ensure an aseptic environment inside the chamber during tissue growth and storage.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.