Detection of nucleic acid sequences by invader-directed cleavage
US5846717A · kind A · utility
397Cited by
16References
32Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 24, 1996 |
| Grant date | Dec 8, 1998 |
| Priority date | — |
| Expiry date | Jan 24, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/822
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The 5' nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.