Opposable-element assay device employing conductive barrier
US5846838A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jun 18, 1997 |
| Grant date | Dec 8, 1998 |
| Priority date | — |
| Expiry date | Jun 18, 2017 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/81
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A chromatographic assay device for use with immunoassays allows rapid and convenient assays of analytes of biological interest, and permits extractions to be carried out in situ, avoiding the use of separate extraction vessels. The device has a wide dynamic range and avoids interference from particulates or colored components. In one form, the device comprises: (1) a first opposable component comprising a sample preparation zone adapted to receive a sample to be assayed; (2) a second opposable component comprising a chromatographic medium; and (3) a conductive barrier attached to the second opposable component. The first and second opposable components can be brought into opposition so as to cause the sample preparation zone to apply the sample to be tested to the chromatographic medium. Preferably, the analyte is detected with a visually detectable label. Other variations of the device vary the arrangement of components to provide optimal chromatography for a variety of analytes. The devices can be incorporated in test kits, and assay methods, particularly sandwich immunoassays, using the devices are also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.