Method for eliciting an immune response using a gene expression system that co-delivers an RNA polymerase with DNA
US5846949A · kind A · utility
8Cited by
3References
13Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 3, 1997 |
| Grant date | Dec 8, 1998 |
| Priority date | — |
| Expiry date | Oct 3, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2830/85
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a gene expression system in eukaryotic cells. In particular, it relates to a self-initiating and self-sustaining gene expression system utilizing an RNA polymerase prebound to a DNA construct before the introduction of the complex into cells. This system is capable of functioning in the cell cytoplasm without integration into the genome of host cells, and does not require host cell factors for initiation and maintenance of gene expression. Therefore, the invention has a wide range of applications in in vitro and in vivo gene expression, including gene therapy in resting cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.