Methods of generating desired amino-terminal residues in peptides
US5847097A · kind A · utility
6Cited by
6References
7Claims
0Family size
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Key dates
| Filing date | Dec 14, 1995 |
| Grant date | Dec 8, 1998 |
| Priority date | — |
| Expiry date | Dec 14, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/95
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods of designing or modifying protein structure at the protein or genetic level to produce specified amino-termini in vivo or in vitro are described. The methods can be used to alter the metabolic stability and other properties of the protein or, alternatively, to artificially generate authentic amino-termini in proteins produced through artificial means. The methods are based upon the introduction of the use of artificial ubiquitin-protein fusions, and the discovery that the in vivo half-life of a protein is a function of the amino-terminal amino acid of the protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.