Fluorescence imaging system having reduced background fluorescence
US5847400A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 30, 1997 |
| Grant date | Dec 8, 1998 |
| Priority date | — |
| Expiry date | Jan 30, 2017 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/6463
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A coaxial illumination and collection laser scanning system designed to provide increased sensitivity by reducing auto-fluorescence while having a substantially uniform detection sensitivity across the field of view of an objective lens by reducing lateral chromatic aberrations at the expense of amplifying axial chromatic aberrations. Axial chromatic aberrations in the system are removed in the path of a retro-beam. A laser is in optical communication with the objective lens. The laser produces a collimated beam of coherent light that is directed by a scanner through the objective lens to illuminate a raster of spots on the sample's surface, thereby stimulating a series of small regions of the sample to emit light. The system may be used as a confocal or non-confocal imaging system.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.