Method and medium for use in detecting E. coli and total coliforms
US5849515A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jan 30, 1997 |
| Grant date | Dec 15, 1998 |
| Priority date | — |
| Expiry date | Jan 30, 2017 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/849
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A selective culture medium which permits simultaneous detection of total coliform and Escherichia coli in a test sample with a single growth phase incubation period. The culture medium includes the required components of: (i) carbon nutrients, (ii) a nitrogen nutrient, (iii) a source of metabolizable potassium, (iv) a source of metabolizable phosphate, (v) vitamins, (vi) minerals, (vii) amino acids, (viii) sodium pyruvate, (ix) a bactericidal system selective for non-coliform bacteria which includes methylene blue, erythromycin and an azide, and (x) a sensible indicator selectively metabolized by Escherichia coli to the exclusion of other coliforms.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.