Method and composition for preserving antigens and nucleic acids and process for utilizing cytological material produced by same
US5849517A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jul 16, 1996 |
| Grant date | Dec 15, 1998 |
| Priority date | — |
| Expiry date | Jul 16, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/108331
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method and composition for fixing and stabilizing tissues, cells, and cell components such that the antigenic sites and nucleic acids are preserved is provided. The fixative employs a formaldehyde donor that is non-toxic, non-flammable, and that stabilizes the cell with minimal damage to and alteration of the cell morphology. The cell antigenic sites are left intact so that studies with monoclonal antibodies may be conducted. Vaccines and related immunotherapeutic methods utilizing antigens stabilized by the fixative of the present invention are also provided. Also disclosed is a method for developing a positive control for test reagents and for test instrumentation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.