Transformation systems for the yeast candida utilis and the expression of heterologous genes therewith
US5849524A · kind A · utility
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101Claims
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Key dates
| Filing date | May 24, 1996 |
| Grant date | Dec 15, 1998 |
| Priority date | — |
| Expiry date | May 24, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/815
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An reproducible transformation system of a yeast of Candida utilis, a process for expressing a heterologous gene in the transformation system, a vector which can be used in the transformation system and the expression method, and a novel DNA group are disclosed. In particular, the process for expressing a heterologous gene in Candida utilis comprises transforming Candida utilis with a vector comprising a drug-resistance marker, a sequence homologous to the chromosomal DNA of the Candida utilis yeast, and the heterologous gene, culturing the transformant, and isolating the expression product of the heterologous gene.
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