Oxidized phospholipid degrading enzyme and gene thereof
US5849557A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 3, 1994 |
| Grant date | Dec 15, 1998 |
| Priority date | — |
| Expiry date | Aug 3, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/18
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An oxidized phospholipid degrading enzyme is provided, which plays an important role in the oxygen stress preventive mechanism in organisms. The enzyme hydrolyzes a 1-acyl-2-.omega.-carboxyfatty acid acyl-3-phosphatidylcholine as a substrate at the 2-ester bond thereof to form 1-acyl-2-lyso-3-phosphatidylcholine. The activity of the enzyme is slightly enhanced by 4 mM calcium chloride. The molecular mass of the enzyme is 95.+-.5 kDa (by gel filtration). The enzyme is composed of three subunits whose molecular masses have been found to be 29 kDa, 30 kDa and 45 kDa, respectively, by SDS-polyacrylamide electrophoresis. A gene coding the enzyme is also provided. This gene is important for the synthesis of the enzyme by genetic engineering.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.