Native-state histoculturing method for skin
US5849579A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | May 3, 1996 |
| Grant date | Dec 15, 1998 |
| Priority date | — |
| Expiry date | May 3, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2503/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods and compositions for a native-state histoculturing system for skin are disclosed. Skin samples are placed on an extracellular support matrix immersed in a medium whereby the internal surface of the skin is adjacent to the matrix and the external surface is exposed above the surface of the medium and the skin maintained in the medium under skin culturing conditions. An extracellular support matrix comprising a collagen-containing gel and homopolysaccharide sponge or a combination matrix of a top layer of a collagen-containing gel and a bottom layer of a homopolysaccharide sponge is also disclosed. Skin toxicity, hair growth, anti-aging and anti-wrinkling assays utilizing the histoculturing system of the present invention are also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.