Method for purifying granulocyte colony stimulating factor
US5849883A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 9, 1996 |
| Grant date | Dec 15, 1998 |
| Priority date | — |
| Expiry date | May 9, 2016 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K38/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Processes for isolating and purifying granulocyte colony stimulating factor (G-CSF) from a G-CSF producing microorganism are disclosed. The simplified processes include steps of lysing the microorganism and separating insoluble material containing G-CSF from soluble proteinaceous material; extracting the material with deoxycholate (optionally); solubilizing and oxidizing the G-CSF in the presence of a denaturant solubilizing agent and an oxidizing agent; removing the denaturant solubilizing agent from the G-CSF; subjecting the G-CSF to ion exchange chromatography; and recovering the purified G-CSF; yet excludes other cumbersome purification steps.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.