Three component chimeric antisense oligonucleotides
US5849902A · kind A · utility
242Cited by
3References
23Claims
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Key dates
| Filing date | Nov 21, 1996 |
| Grant date | Dec 15, 1998 |
| Priority date | — |
| Expiry date | Nov 21, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/346
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention relates to antisense oligonucleotides that target mRNAs in cells as substrates for the cellular enzyme RNase H and thereby cause specific degradation of the targeted mRNA. The oligonucleotides have three components: a RNase H activating region, a complementarity region and 3' and 5' ends. The invention optimizes each of the components to resist intracellular nucleases, to increase hybridization to target mRNA, to specifically inactivate target mRNA in cells, and to decrease cytotoxicity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.