Method for eliminating the displacer in displacement chromatography of proteins
US5851400A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 21, 1997 |
| Grant date | Dec 22, 1998 |
| Priority date | — |
| Expiry date | Mar 21, 2017 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N30/34
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides a method for eliminating the use of a displacer in displacement chromatography of proteins. Elimination of displacer is accomplished by producing an appropriate retained pH gradient using adsorbed buffering species. When the band velocity curves of the proteins under consideration intersect a vertical section of the pH profile and none of these protein have adsorption isotherm which cross each other at the pH of the intermediate plateau, and when the amount of protein in the feed slug to the column is such that bands of the appropriate concentration are formed in the displacement train, then a displacement pattern results in a chromatography column even though no displacer is present.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.