Analyte assay using a trifunctional conjugate
US5851778A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 2, 1997 |
| Grant date | Dec 22, 1998 |
| Priority date | — |
| Expiry date | Apr 2, 2017 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/816
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A trifunctional conjugate is provided having three chemical moieties attached through a spacer moeity. At least two of the chemical moieties are relatively small molecules, usually less than about 7,000 Daltons in size. The spacer moiety is selected to impart certain steric properties to the conjugate. In one embodiment, the binding of a macromolecular specific binding partner to one of the chemical moieties sterically inhibits the binding of a different macromolecule to another chemical moiety. In another embodiment, the binding of a first chemical moiety to a macromolecule restricts the subsequent binding of a second chemical moiety to a proximate location on the same macromolecule. The three chemical moieties are preferably a nitrophenylazido residue, a phenyl boronic acid residue, and a solid support or a label such as biotin. The spacer is preferably cysteine, lysine, glutamic acid, pyroglutamic acid, S-acetylmercaptosuccinic anhydride or .omega.-carbobenzoxylysine. The conjugate is useful in immunoassays and for targeted labeling of proteins. In an assay, a sample is contacted with the conjugate, a limited quantity of analyte binding partner and an excess of small molecule bi…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.