Molecular cloning of the ovine pituitary growth hormone releasing hormone receptor
US5852187A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 12, 1996 |
| Grant date | Dec 22, 1998 |
| Priority date | — |
| Expiry date | Jun 12, 2016 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/726
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Growth hormone releasing hormone (GHRH) receptor binding has been characterized using a unique binding assay utilizing iodinated GHRH probes. Photoaffinity GHRH probes have been constructed which allow for photolabeling and characterization of the receptor. In addition, high affinity biotinylated GHRH analogs have been constructed. Solubilization of GHRH-R/GHRH complexes and extraction of specifically bound GHRH using a mild detergent solution, followed by affinity chromatography, leads to a substantially purified GHRH-R isolate. Electrophoretic treatment of the GHRH-R isolate produces GHRH-R of sufficient purity to conduct sequencing of the receptor. Cloning of a gene encoding for polypeptides (protein or fragments thereof) having GHRH-R activity is accomplished using a bacterial host, and the cloned gene is expressed in a mammalian cell line. In addition, molecular cloning of the ovine GHRH-R is provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.