Recombinant proteinase from clostridium histolyticum and its use for isolating cells and groups of cells

US5853976A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Friederike Hesse · Munich, DE
• Dorothee Ambrosius · Munich, DE
• Helmut Burtscher · Habach, DE

Key dates

• Filing date: Aug 26, 1997
• Grant date: Dec 29, 1998
• Expiry date: Aug 26, 2017

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61P37/02
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Process for disintegrating cell tissue and releasing cells or groups of cells contained therein by incubating the cell tissue with a recombinant neutral protease from Clostridium histolyticum which is coded by PA1 a) a DNA of nucleotides 1027-1965 from SEQ ID NO:3 or a DNA which is complementary thereto, PA1 b) nucleic acids which hybridize with a DNA of nucleotides 1027-1965 from SEQ ID NO:3, PA1 c) nucleic acids which, without the degeneracy of the genetic code, would hybridize with one of the nucleic acids mentioned in a) or b) and is the product of a prokaryotic or eukaryotic expression of an exogenous nucleic acid, until the cells or groups of cells have been released to the desired extent and separating the cells or groups of cells from the cell tissue fractions.