Chemical promotion of nucleic acid hybridization
US5853986A · kind A · utility
6Cited by
4References
8Claims
0Family size
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Key dates
| Filing date | Jun 13, 1995 |
| Grant date | Dec 29, 1998 |
| Priority date | — |
| Expiry date | Jun 13, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6832
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An improved method is described for increasing the rate and specificity of hybridization of target polynucleotide sequences with complementary probes. The method uses amphipathic hydrocarbon polymers (AHP), exemplified by polyvinyl sulfonic acid or polystyrene sulfonic acid, to effect the improved hybridization characteristics. The method may be used in solution phase, solid phase, or in situ hybridization formats.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.