Process for screening substances capable of modulating a receptor-dependent cellular signal transmission path

US5854004A · kind A · utility

Assignee

• Boehringer Ingelheim GmbH · DE

Inventors

• Armin Peter Czernilofsky · Wien, AT
• Adolf Himmler · Wien, AT
• Christian Stratowa · Wien, AT
• Ulrike Weyer · Wien, AT
• Herbert Lamche · Alland, AT
• Renate Schafer · Wien, AT

Key dates

• Filing date: May 25, 1994
• Grant date: Dec 29, 1998
• Expiry date: May 25, 2014

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/5008
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A process is disclosed for screening substances having a modulating effect on a receptor-dependent signal transmission path in mammal cells. Test cells are used transformed with a reporter gene and with a regulatory sequence functionally linked thereto sensitive to the IP.sub.3 /DAG concentration, as well as with a coding DNA for a receptor coupled to the phospholipase effector system, in particular a G protein-coupled receptor. The use of reference cells without receptor DNA and reference cells with specificity for the adenylate cyclase effector system allows substances to be identified having potential pharmacological action and specificity for a determined receptor-dependent signal transmission path.