Patent · US Expired

Hexokinase inhibitors

US5854067A · kind A · utility

53Cited by
5References
64Claims
0Family size

Assignees

Inventors

Key dates

Filing dateJan 19, 1996
Grant dateDec 29, 1998
Priority date
Expiry dateJan 19, 2016

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N2310/121
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Disclosed are compositions and methods for inhibiting hexokinase enzymes in mammalian cells. Specifically provided are proteins that stimulate the production of trehalose-6-phosphate and their respective genes; hexokinase-specific ribozymes and genes encoding such constructs; and agents that competitively reduce hexokinase activity, e.g., by displacing hexokinase from mitochondria, and their respective genes. The latter group of agents includes inactive hexokinases and fragments thereof that retain mitochondrial binding functions and hexokinase-glucokinase chimeras that further substitute glucokinase activity for hexokinase activity. Mammalian cells including such hexokinase inhibitors, methods of making such cells and various in vitro and in vivo methods of using cells with reduced hexokinase activity are also described herein.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.