Method for isolation of highly pure von Willebrand Factor

US5854403A · kind A · utility

Assignee

• Immuno Aktiengesellschaft · AT

Inventors

• Bernhard Fischer · Wien, AT
• Artur Mitterer · Orth an der Donau, AT
• Friedrich Dorner · Wien, AT
• Hans-Peter Schwarz · Wien, AT
• Peter Turecek · Klosterneuburg, AT
• Johann Eibl · Wien, AT
• Falko-Guenter Falkner · Orth an der Donau, AT
• Uwe Schlokat · Orth an der Donau, AT
• Wolfgang Mundt · Wien, AT
• Manfred Reiter · Wien, AT
• Renate Den-Bouwmeester · Wien, AT

Key dates

• Filing date: May 24, 1996
• Grant date: Dec 29, 1998
• Expiry date: May 24, 2016

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K38/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The invention relates to a method for isolation of highly pure von Willebrand Factor in which recombinant von Willebrand Factor (rvWF) is chromatographically purified by anion exchange chromatography on an anion exchanger of the quaternary amino type in a buffer solution comprising buffer substances and optionally salt. The buffer solutions are preferably free of stabilizers, amino acids and other additives. According to this method, highly pure recombinant rvWF can be obtained, which is free from blood plasma proteins, especially free from Factor VIII, and is physiologically active. Further, the invention relates to a pharmaceutical preparation that contains rvWF, which comprises mulitimers with a high structural integrity.