Lawn assay for compounds that affect enzyme activity or bind to target molecules
US5856083A · kind A · utility
65Cited by
9References
22Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 3, 1995 |
| Grant date | Jan 5, 1999 |
| Priority date | — |
| Expiry date | Nov 3, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B40/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A lawn assay is described for determining compounds that affect enzyme activity or that bind to target molecules. Compounds to be screened are cleaved, and diffused from solid supports into a colloidal matrix. Enzymatic catalysis or binding to target molecules by the compounds is carried out in the matrix. Active compounds are found by monitoring a photometrically detectable change in a substrate, coenzyme, or cofactor involved in the enzymatic reaction, or in a labeled ligand bound to the target molecule, that produces a zone of activity associated with the compounds.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.