Direct cloning of DNA fragments
US5856144A · kind A · utility
18Cited by
1References
12Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 18, 1997 |
| Grant date | Jan 5, 1999 |
| Priority date | — |
| Expiry date | Jun 18, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/66
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A vector for the direct cloning of the products of PCR protocol incorporates single nucleotide overhangs at one or both ends of a linearized DNA segment. The single nucleotide overhangs are uracil or inosine residues, as desired, to facilitate cloning of the desired PCR products.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.