Purified nitric oxide synthase from rat brain
US5856158A · kind A · utility
8Cited by
3References
7Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jul 5, 1996 |
| Grant date | Jan 5, 1999 |
| Priority date | — |
| Expiry date | Jul 5, 2016 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K38/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A nitric oxide synthase (NOS) protein is obtained from rat brain and purified to a specific activity at least 6,360-fold that of a crude tissue preparation. The NOS protein has a denatured MW of 105 kD determined by SDS-PAGE, a dimeric MW of 230 kD determined by gel filtration and requires FAD, FMN, Ca.sup.2+ and tetrahydropterin for the production of nitric oxide from L-arginine or an arginine-rich peptide, oligopeptide or protein substrate.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.