Precision and accuracy of anion-exchange separation of nucleic acids
US5856192A · kind A · utility
20Cited by
15References
10Claims
0Family size
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Key dates
| Filing date | Jan 23, 1996 |
| Grant date | Jan 5, 1999 |
| Priority date | — |
| Expiry date | Jan 23, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Solvents for salt-gradient anion-exchange separation of nucleic acids, especially double-stranded DNA and especially by liquid chromatography, are improved by replacing NaCl as the eluting salt with any of a wide range of alkyl ammonium salts and can be used to elute nucleic acids in strict order of increasing length, with reduced sensitivity to elution temperature and salt concentration. Anion-exchange chromatography with these solvents is well suited for identification of DNA fragments on the basis of size, with greater accuracy, precision, and resolvable size range than often is possible with gel electrophoresis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.