Iterative and regenerative DNA sequencing method
US5858671A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Nov 1, 1996 |
| Grant date | Jan 12, 1999 |
| Priority date | — |
| Expiry date | Nov 1, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6855
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An iterative and regenerative method for sequencing DNA is described. This method sequences DNA in discrete intervals starting at one end of a double stranded DNA segment. This method overcomes problems inherent in other sequencing methods, including the need for gel resolution of DNA fragments and the generation of artifacts caused by single-stranded DNA secondary structures. A particular advantage of this invention is that it can create offset collections of DNA segments and sequence the segments in parallel to provide continuous sequence information over long intervals. This method is also suitable for automation and multiplex automation to sequence large sets of segments.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.