Method for determining the presence of functional p53 by measuring GADD45 protein expression
US5858679A · kind A · utility
Inventors
Key dates
| Filing date | May 10, 1995 |
| Grant date | Jan 12, 1999 |
| Priority date | — |
| Expiry date | May 10, 2015 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/47
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The dependence of ionizing radiation-induced GADD45 mRNA and protein expression on the presence of functional p53 in mammalian cells is disclosed. First and second oligonucleotide sequences are provided which can form a double-stranded oligomer capable of binding to functional p53 protein. The present invention demonstrates that the dependence of ionizing radiation-induced GADD45 mRNA and protein expression on the presence of functional p53 and the binding of functional p53 to a double-stranded oligomer binding sequence can serve as the bases for methods for determining the presence of functional p53 in mammalian cell lines and tumors.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.