Method and agent for the simultaneous colorimetric and electrochemical measurement of an analyte

US5858691A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Joachim Hoenes · Zwingenberg, DE
• Holger Kotzan · Ladenburg, DE
• Volker Unkrig · Ladenburg, DE
• Michael Marouant · Mannheim, DE

Key dates

• Filing date: Jun 13, 1996
• Grant date: Jan 12, 1999
• Expiry date: Jun 13, 2016

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/26
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention concerns a method for the simultaneous colorimetric and electrochemical measurement of an analyte using an oxidizing enzyme and a chromogen A that accepts electrons from the enzyme which is characterized in that the chromogen A after reduction to A' reacts by coupling to a substance BX to form a colored substance A'B the concentratin of which is measured colorimetrically as a measure for the presence or amount of the analyte and in that an electrochemically measurable group X.sup.- is cleaved off during the coupling reaction the concentration of which is measured electrochemically as a measure for the amount of the analyte.