Recombinant rabbit tissue factor
US5858724A · kind A · utility
9Cited by
8References
11Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jul 16, 1996 |
| Grant date | Jan 12, 1999 |
| Priority date | — |
| Expiry date | Jul 16, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/745
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Recombinant rabbit tissue factor is cloned and produced in a bacterial host. This protein, which is relatively insoluble and has several disulfide bonds, requires special modifications in order to express and be purified at commercial levels. By expressing the tissue factor as a fusion protein with a bacterial enzyme, thioredoxin, solubility is increased. Use of a thioredoxin reductase deficient host aids in proper tertiary structure for biological activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.