Control of cell growth in a bioartificial organ with extracellular matrix coated microcarriers

US5858747A · kind A · utility

Assignee

• Cytotherapeutics, Inc. · US

Inventors

• Malcolm Schinstine · Lehighton, US
• Molly S. Shoichet · Toronto, CA
• Frank T. Gentile · East Greenwich, US
• Joseph P. Hammang · Barrington, US
• Laura M. Holland · Ambler, US
• Brian M. Cain · Everett, US
• Edward J. Doherty · Halifax, US
• Shelley R. Winn · Providence, US
• Patrick Aebischer · Lutry, CH

Key dates

• Filing date: May 23, 1995
• Grant date: Jan 12, 1999
• Expiry date: May 23, 2015

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2533/74
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Methods and compositions are provided for controlling cell distribution within an implantable bioartificial organ by exposing the cells to a treatment that inhibits cell proliferation, promotes cell differentiation, or affects cell attachment to a growth surface within the bioartificial organ. Such treatments include (1) genetically manipulating cells, (2) exposing the cells to a proliferation-inhibiting compound or a differentiation-inducing compound or removing the cells from exposure to a proliferation-stimulating compound or a differentiation-inhibiting compound; exposing the cells to irradiation, and (3) modifying a growth surface of the bioartificial organ with extracellular matrix molecules, molecules affecting cell proliferation or adhesion, or an inert scaffold, or a combination thereof. These treatments may be used in combination. The bioartificial organ typically has a semipermeable membrane encapsulating a cell-containing core, and is preferably immunoisolatory. Cells can be grown on microcarriers and then loaded into the bioartificial organ. The microcarriers may be coated with an extracellular matrix component such as collagen to cause decreased cell proliferation or …