Production of normal mammalian organ culture using a medium containing mem-alpha, leibovitz L-15, glucose galactose fructose

US5858783A · kind A · utility

Assignee

• The United States of America as represented by the Administrator of the National Aeronautics and Space Administration · US

Inventors

• Thomas J. Goodwin · Friendswood, US
• David A. Wolf, Sr. · Houston, US
• Glenn F. Spaulding · Houston, US
• Tacey L. Prewett · Friendswood, US

Key dates

• Filing date: Mar 1, 1996
• Grant date: Jan 12, 1999
• Expiry date: Mar 1, 2016

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2500/72
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Normal mammalian tissue and the culturing process has been developed for the three groups of organ, structural and blood tissue. The cells are grown in vitro under micro-gravity culture conditions and form three dimensional cells aggregates with normal cell function. The microgravity culture conditions may be microgravity or simulated micro-gravity created in a horizontal rotating wall culture vessel. The medium used for culturing the cells, especially a mixture of epithelial and mesenchymal cells contains a mixture of Mem-alpha and Leibovits L15 supplemented with glucose, galactose and fructose.