Activated esters of 1-phenylpyrazolin-5-one for labeling amine-functionalized molecules
US5859259A · kind A · utility
1Cited by
5References
6Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 15, 1996 |
| Grant date | Jan 12, 1999 |
| Priority date | — |
| Expiry date | Aug 15, 2016 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/533
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The disclosed invention is drawn to pyrazolinone derivatives, and methods of use thereof, for non-radioactive labeling of amine-functionalized molecules, especially biomolecules such as DNA, RNA, PNA, oligomers, carbohydrates, amino acids and peptides. Carboxyl-containing reporter groups such as biotin and fluorescein may be activated for nucleophilic addition to a primary or secondary amine moiety. Representative compounds of the invention include Dimethoxytritylbiotin-XPP, biotin-XPP, and fluorescein-XPP.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.