Protecting nucleic acids and methods of analysis
US5861250A · kind A · utility
10Cited by
3References
23Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 6, 1996 |
| Grant date | Jan 19, 1999 |
| Priority date | — |
| Expiry date | Jun 6, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6816
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A selected region of a nucleic acid is protected from attach by nuclease by completing to the nucleic acid a nucleic acid analogue of the PNA type. The surviving sequence may be detected in an assay, optionally after amplification. A PCR reaction may be sterilized and its product assayed by protecting a characterizing region of the product by PNA hybridization followed by nuclease degradation of unprotected nucleic acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.