Enzymatic method for detecting coliform bacteria or E. coli
US5861270A · kind A · utility
Assignees
Inventor
Key dates
| Filing date | Jul 14, 1997 |
| Grant date | Jan 19, 1999 |
| Priority date | — |
| Expiry date | Jul 14, 2017 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/968
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A two stage enzymatic method for the detection of coliform bacteria or E. coli wherein bacteria are concentrated on a membrane filter. This filter is placed on a growth medium containing nutrients, including preferably minerals, a protein hydrolysate and a sugar, preferably maltose or a polyalcohol, preferably mannitol, an inducer of a marker enzyme, in particular .beta.-galactosidase or .beta.-glucuronidase and inhibitors of the growth of competing bacteria. After a preincubation step, the filter is placed on an assay medium containing a fluorogenic or chemiluminogenic enzyme substrate and a membrane permeabilizer. The membrane filter and the assay medium are incubated to allow cleavage of the enzyme substrate producing fluorescent or chemiluminescent microcolonies on the membrane filter after triggering of light emission.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.