Baculovirus expression system for human interleukin 5 receptor and method of screening for interleukin 5 antagonists
US5861279A · kind A · utility
83Cited by
4References
9Claims
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Key dates
| Filing date | Jan 11, 1997 |
| Grant date | Jan 19, 1999 |
| Priority date | — |
| Expiry date | Jan 11, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2710/14143
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A baculovirus/insect cell expression system in which both .alpha. and .beta. subunit of human IL-5 receptor can be over-expressed together in Sf9 cells has been developed. This system is able to express the human high-affinity IL-5 receptors at a very high level and finds use in a screening assay for IL-5 receptor-based antagonists. A preferred embodiment of the screening assay involves the use of peanut agglutinin as lectin in a scintillation proximity assay.
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