Genetic construct of which protein coding DNA comprises introns and is designed for protein production in transgenic animals
US5861299A · kind A · utility
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16Claims
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Key dates
| Filing date | Nov 17, 1994 |
| Grant date | Jan 19, 1999 |
| Priority date | — |
| Expiry date | Nov 17, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2830/85
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Proteinaceous products can be produced by transgenic animals having genetic constructs integrated into their genome. The construct comprises a 5'-flanking sequence from a mammalian milk protein gene (such as beta-lactoglobulin) and DNA coding for a heterologous protein other than the milk protein (for example a serin protease such as alpha.sub.1 -antitrypsin or a blood factor such as Factor VIII or IX). The protein-coding DNA comprises at least one, but not all, of the introns naturally occurring in a gene coding for the heterologous protein. The 5'-flanking sequence is sufficient to drive expression of the heterologous protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.