Production in yeasts of stable antibody fragments
US5863765A · kind A · utility
53Cited by
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13Claims
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Key dates
| Filing date | Nov 12, 1997 |
| Grant date | Jan 26, 1999 |
| Priority date | — |
| Expiry date | Nov 12, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Associative portions of antibody light and heavy chains, especially Fv fragments, are expressed in a transformed organism as a single peptide chain connected by a linking peptide. This is cleaved, possibly while peptide synthesis is incomplete, by an enzyme of the transformed organism which is expressing the single peptide. This ensures production of both chains in equal amounts, but produces them as separate chains which are free to associate and fold.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.