Method for collecting hemolymph of insects
US5866317A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 15, 1997 |
| Grant date | Feb 2, 1999 |
| Priority date | — |
| Expiry date | Jul 15, 2017 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01K67/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention herein provides a method for collecting insect hemolymph which permits the collection of hemolymph, at a time, from a vast number of insect bodies, which does not cause any scattering of the hemolymph during collection thereof, which may widely be used, which permits the collection of the hemolymph free of unnecessary tissues or the like and which can inhibit the melanization of the collected hemolymph. The insect hemolymph can be collected by freezing anesthetized lepidopterous insects, piercing the epidermis of the frozen insects without damaging the alimentary canals thereof and then thawing them in a buffer solution containing a melanization-inhibitory agent to thus discharge the hemolymph of the insects into the buffer solution through the holes while making use of the self-contraction phenomenon caused during the thawing process.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.