Method for determination of glucose in whole blood and cuvette and photometer for carrying out said method
US5866349A · kind A · utility
Inventors
Key dates
| Filing date | Oct 16, 1991 |
| Grant date | Feb 2, 1999 |
| Priority date | — |
| Expiry date | Oct 16, 2011 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2201/126
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A sample of whole blood is contacted with a reagent which by chemical reaction with glucose in the sample brings about a detectable dye concentration change (10) the size of which is determined as a measure of the glucose content of the sample. The sample is initially introduced undiluted in a microcuvette having at least one cavity for receiving the sample. The cavity is internally pretreated with the reagent in dry form, and the chemical reaction takes place in the cavity. Active components of the reagent comprise at least a hemolyzing agent for exposing glucose contained in the blood cells of the sample for allowing total glucose determination, and agents taking part in the chemical reaction and ensuring that the dye concentration change (10) takes place at least in a wavelength range (14) outside the absorption range (12) of the blood hemoglobin. An absorption measurement is performed in said wavelength range directly on the sample in the cuvette. A pretreated disposable cuvette with such a reagent and a photometer are also described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.