Electro chemical biosensor containing diazacyanine mediator for co-enzyme regeneration

US5866353A · kind A · utility

Assignee

• BAYER HEALTHCARE LLC · DE

Inventors

• Horst Berneth · Leverkusen, DE
• Thomas Böcker · Leichlingen, DE
• Henry Giera · Wittlich, DE
• Alison J. Murray · Elkhart, US
• Hans-Ulrich Siegmund · Cologne, DE

Key dates

• Filing date: Dec 9, 1996
• Grant date: Feb 2, 1999
• Expiry date: Dec 9, 2016

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/817
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A variety of diazacyanine mediators that are soluble in aqueous media and which do not inhibit enzymatic activity are provided for use on the surface of a working electrode of a electrochemical biosensor for electrochemical co-enzyme regeneration. The co-enzyme, dihydronicotinamide adenine dinucleotide (NADH) or dihydronicotinamide adenine dinucleotide phosphate (NADPH), is oxidized to NAD.sup.+ OR NADP.sup.+ which is reduced by an oxidoreductase such as a dehydrogenase acting on a substrate. By applying the mediator together with NADH or NADPH to the surface of the working electrode, the voltage necessary to achieve oxidation is substantially reduced. Biosensor electrodes such as graphite electrodes may be produced-by screen printing techniques.