Device for measuring an intracellular ion concentration
US5866355A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Jun 3, 1996 |
| Grant date | Feb 2, 1999 |
| Priority date | — |
| Expiry date | Jun 3, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/973
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A device for measuring an intracellular ion concentration in a living cell into which a fluorescent probe dye has been introduced. A concentration of an ion in the cell is measured based on intensities of fluorescence generated by irradiating the cell with excitation beams, and comparing the intensities of fluorescence emitted by the cell with fluorescence intensities generated from each of 3 reference solutions irradiated with excitation beams. The device is composed of a container means for holding an object to be measured, an excitation beam irradiating means, a fluorescence intensity detecting means, a first processing means for receiving output signals of the fluorescence intensity detecting means and a second processing means for solving simultaneous equations to determine a concentration of the ion to be measured within the cell.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.