Method for hydrolyzing proteins with gluyasp specific protease
US5866357A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 31, 1992 |
| Grant date | Feb 2, 1999 |
| Priority date | — |
| Expiry date | Aug 31, 2012 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA23J3/34
- WIPO fieldFood chemistry
- WIPO sectorChemistry
Abstract
A method is disclosed for obtaining limited specific hydrolysis of proteins using a Glu/Asp specific protease to produce peptides with C-terminal Glu or Asp. The protease is obtained from Bacillus licheniformis, has a mass of about 23,600, is inhibited by diisopropyl fluoride but not by phenylmethane sulfonylfluoride, and has a pH of maximal activity between 6.5 and 10.0. The proteins are treated with the protease at neutral pH and the reaction terminated by raising the temperature above 70.degree. C. or lowering the pH below 5.0 to obtain the hydrolysate. The process may include a second protease which is specific for Arg/Lys, and produces peptides with C-terminal Arg or Lys.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.