Process for the synthesis of nucleotide-6-deoxy-D-xylo-4-hexuloses

US5866378A · kind A · utility

Assignee

• Hoechst GmbH · DE

Inventors

• Ruediger Marquardt · Frankfurt, DE
• Brigitte Hoersch · Kriftel, DE
• Andreas Seiffert-Stoeriko · Frankfurt, DE
• Andreas Stein · Schneverdingen, DE
• Astrid Zervosen · Welkenraedt, BE
• Lothar Elling · Aachen, DE
• Maria-Regina Kula · Jülich, DE
• Stefan Verseck · Hanau, DE
• Juergen Distler · Wuppertal, DE
• Wolfgang Piepersberg · Wuppertal, DE

Key dates

• Filing date: Oct 7, 1996
• Grant date: Feb 2, 1999
• Expiry date: Oct 7, 2016

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07H19/04
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

The present invention relates to processes for the enzymatic synthesis of nucleotide-6-deoxy-D-xylo-4-hexuloses starting from a nucleoside monophosphate (NMP). These processes comprise simultaneous incubation of the following substances in a buffer solution: PA1 (a) substrates comprising a nucleoside monophosphate, phosphoenolpyruvate, adenosine triphosphate, and sucrose; and PA1 (b) enzymes comprising pyruvate kinase, nucleoside-monophosphate kinase, sucrose synthase and deoxythymidine-D-glucose 4,6dehydratase.