Use of primers containing nucleotides having altered base pairing characteristics in the amplification of nucleic acid molecules
US5869251A · kind A · utility
14Cited by
2References
17Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 25, 1996 |
| Grant date | Feb 9, 1999 |
| Priority date | — |
| Expiry date | Nov 25, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/156
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides improved methods for amplifying a nucleic acid molecule. More specifically, the invention involves replacing at least one nucleotide of an oligonucleotide with a nucleotide having altered base pairing characteristics, so as to more equalize the efficiency with which that oligonucleotide and a second oligonucleotide hybridize to a target molecule and then amplifying the target molecule using, for example, the polymerase chain reaction. Improved amplification results from the improvement in relative hybridization efficiencies.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.