Affinity ultrafiltration assay for transferase activity
US5869275A · kind A · utility
Inventor
Key dates
| Filing date | Jul 20, 1998 |
| Grant date | Feb 9, 1999 |
| Priority date | — |
| Expiry date | Jul 20, 2018 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/975
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
The present invention relates to a method for assaying transferase activity upon incorporation of an affinity ultrafiltration process as a separation means, which method comprises: (1) reacting the transferase to be assayed with a labeled substrate and an unlabeled substrate to yield a product having a label moiety from the labeled substrate and a binding site moiety either from the unlabeled substrate or existing as a specific structure of the product, (2) contacting the reaction mixture with a soluble macroligand capable of forming a specific complex with the product via the binding site moiety, (3) subjecting the complex mixture to ultrafiltration which retains the complex and passes the unreacted labeled substrate, (4) washing the retentate, and (5) determining the final retentate. The present invention also relates to a kit embodying the inventive concept of the affinity ultrafiltration assay for transferase activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.